Morphometric and molecular sequencing evidence for the identification of Artemia franciscana (Kellogg, 1906) in Southern India

Authors

  • Y. Biju Center for Marine Science and Technology, Manonmaniam Sundaranar University
  • S. Krishnakumar Department of Biomedical Engineering, School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology
  • M. Krishnaveni Center for Marine Science and Technology, Manonmaniam Sundaranar University

DOI:

https://doi.org/10.22364/eeb.21.03

Keywords:

Artemia, cytochrome c oxidase I gene, DNA sequencing, morphometric analysis, solar saltpan

Abstract

A native of North America, the brine shrimp Artemia franciscana is now a very invasive foreign species that has already been imported to most of the world. Before the introduction of A. franciscana, Asia was home to a wide range of bisexual Artemia species, which is no longer the current case. The collected Artemia samples from salt pans in south India were subjected to exact species identification and determination of phylogeographic origin through morphometric and molecular phylogenetic analysis. The Artemia species was validated with the observation of spiny subspherical frontal knob and distinctive lateral triangular ovisac lobes with projections on its body. The phylogenetic analysis of these Artemia species from two sampling localities was carried out after nucleic acid sequencing. After DNA barcoding of the cytochrome oxidase COI gene, the samples were identified as belonging to A. franciscana.

References

Abatzopoulos T.J., Marian P.M. 2004. Characterization of a new parthenogenetic Artemia population from Thamaraikulam, India. J. Biol. Res. 2: 63–74.

Ahmed H.O., Fatma A., Razek A., Khafage A.R., Sarhan M., Soliman T., Aly H., El-Deeb R.S., Al-Aziz E.S.A. 2021. A new record of the Jinga shrimp Metapenaeus affinis (H. Milne Edwards, 1837) (Decapoda, Penaeidae), on the Egyptian coasts, the Mediterranean Sea. Egypt. J. Aquat. Res. 47: 143–150.

Asem A., Eimanifer A., Sun S.C. 2016. Genetic variation and evolutionary origins of parthenogenetic Artemia (Crustacea: Anostraca) with different ploidies. Zool. Scripta 45: 421–436.

Asem A., Wang P., Sun S.C. 2018. Comparative phylogenetic perspectives on the evolutionary relationships in the brine shrimp Artemia Leach, 1819 (Crustacea: Anostraca) based on secondary structure of ITS1 gene. J. Gen. Resour. 4: 72–84.

Basbug Y., Demirkalp F.Y. 1997. A note on the brine shrimp Artemia in Tuz Lake (Turkey). Hydrobiologia 353: 45–51.

Biju Y., Krishnaveni M., Babu M., Krishnakumar S. 2020. Influence of physicochemical parameters on Artemia population in solar saltpans of Kanyakumari District, Tamilnadu, India. Int. J. Psychosoc. Rehab. 24: 4016–4028.

Browne R.A., Bowen S.T. 1991. Taxonomy and population genetics of Artemia. In: Browne R.A., Sorgeloos P., Trotman C.N.A. (Eds.) Artemia Biology. CRC Press, Boca Rato, pp. 221–235.

David P., Thebault E., Anneville O., Duyck P.F., Chapuis E., Loeuille N. 2017. Impacts of invasive species on food webs: a review of empirical data. Adv. Ecol. Res. 56: 1–60.

Dereeper A. Guignon V., Blanc G., Audic S., Buffet S., Chevenet F., Dufayard J.F., Guindon S., Lefort V., Lescot M., Claverie J.M., Gascuel O. 2008. Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 36: W465–W469.

Edgar R.C., 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32: 1792–1797.

Eimanifar A., Van Stappen G., Marden B., Wink M. 2014. Artemia biodiversity in Asia with the focus on the phylogeography of the introduced American species Artemia franciscana Kellogg, 1906. Mol. Phylogenet. Evol. 79: 392–403.

El-Banhawy M.A., El-Gansory M.A. 1989. Microscopic Techniques, Histology, Cytology and Histochemistry. Dar El Marif Publisher, Cairo, 233 p.

El-Sharawy R.T., Elkhateeb A., Marzouk M.M., El-Latif R.A.A., Abdelrazig S.E., El-Ansari MA. 2017. Antiviral and antiparasitic activities of clovamide: the major constituent of Dichrostachys cinerea (L.) Wight et Arn. J. Appl. Pharmac. Sci. 7: 219–223.

Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R., 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Marine Biol. Biotechnol. 3: 294–299.

Galal-Khallaf A., Ardura A., Mohammed-Geba K., Borrell Y.J., Garcia-Vazquez E. 2014. DNA barcoding reveals a high level of mislabelling in Egyptian fish fillets. Food Contr. 46: 441–445.

Krishnakumar V., Munuswamy N. 2014. Occurrence of morphotypes in the invader species, Artemia franciscana Kellogg, 1906 (Crustacea: Anostraca) from Covelong salt works, South India. Int. J. Adv. Res. 2: 1157–1167.

Lee C.E., 2002. Evolutionary genetics of invasive species. Trends Ecol. Evol. 17: 386–391.

Lenormand T., Nougué O., Jabbour-Zahab R., Arnaud F., Dezileau L., Chevin L.-M., Sánchez M.I. 2018. Resurrection ecology in Artemia. Evol. Applic. 11: 76–87.

McMahon R.F. 2002. Evolutionary and physiological adaptations of aquatic invasive animals: R selection versus resistance. Can. J. Fish. Aquat. Sci. 59: 1235–1244.

Morrison W.E., Hay M.E. 2011. Herbivore preference for native vs. exotic plants: generalist herbivores from multiple continents prefer exotic plants that are evolutionarily naive. PLoS One 6: 17227.

Munoz J., Gómez A., Figuerola J., Amat F., Rico C., Green A.J. 2014. Colonization and dispersal patterns of the invasive American brine shrimp Artemia franciscana (Branchiopoda: Anostraca) in the Mediterranean region. Hydrobiologia 726: 25–41.

Mura G., Kappas I., Baxevanis A.D., Moscatello S., D’Amico Q., Lopez G.M., Hontoria F., Amat F., Abatzopoulos T.J. 2006. Morphological and molecular data reveal the presence of the invasive Artemia franciscana in Margherita di Savoia salterns (Italy). Int. Rev. Hydrobiol. 91: 539–554.

Naganawa H., Mura G. 2017. Two new cryptic species of Artemia (Branchiopoda, Anostraca) from Mongolia and the possibility of invasion and disturbance by the aquaculture industry in East Asia. Crustaceana 90: 1679–1698.

Naihong X., Audenaert E., Vanoverbeke J. 2000. Low among-population genetic differentiation in Chinese bisexual Artemia populations. Heredity 84: 238–243.

Ruebhart D.R., Cock I.E., Shaw G.R. 2008. Invasive character of the brine shrimp Artemia franciscana Kellogg 1906 (Branchiopoda: Anostraca) and its potential impact on Australian inland hypersaline waters. Mar. Freshwater Res. 59: 587–595.

Saad El-Sadek Y.M., Saad H.E.A. El-Sadek. 2017. The efficiency of cytochrome oxidase subunit 1 gene (cox1) in reconstruction of phylogenetic relations among some Crustacean species. Int. Schol. Sci. Res. Innov. 11: 515–520.

Sanchez M.I., Paredes I., Lebouvier M., Green A.J. 2016. Functional role of native and invasive filter-feeders, and the effect of parasites: Learning from hypersaline ecosystems. PLoS One 11: 0161478.

Scalone R., Rabet N. 2013. Presence of Artemia franciscana (Branchiopoda, Anostraca) in France: morphological, genetic, and biometric evidence. Aquatic Invas. 8: 67–76.

Sheir S.K., Galal-Khallaf A., Mohamed A.H., Mohammed-Geba K. 2018. Morphological and molecular clues for recording the first appearance of Artemia franciscana (Kellogg, 1906) in Egypt. Heliyon 4: 1110.

Talavera G., Castresana J. 2007. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst. Biol. 56: 564–577.

Tanguay J.A., Reyes R.C., Clegg J.S. 2004. Habitat diversity and adaptation to environmental stress in encysted embryos of the crustacean Artemia. J. Biosci. 29: 489–501.

Van Stappen G. 2002. Zoogeography. In Abatzopoulos T.J., Beardmore J.A., Clegg J.S., Sorgeloos P. (Eds.) Artemia: Basic and Applied Biology. Kluwer Academic Publishers, Dordrecht, pp. 171–224

Van Stappen G., Fayazi G., Sorgeloos P. 2001. Field study of the Artemia urmiana (Gunther, 1890) popupation in Lake Urmiah, Iran (International study on Artemia LXIII). Hydrobiologia 466: 133–143.

Van Stappen G., Sui L., Hoa V. N., Tamtin M., Nyonje B., Rocha R. M., Sorgeloos P and Gajardo G., 2020. Review on integrated production of the brine shrimp Artemia in solar salt ponds. Rev. Aquacult. 12: 1054–1071.

Vetriselvan M., Munuswamy N. 2011. Morphological and molecular characterization of various Artemia strains from tropical saltpans in South East Coast of India. J. Amer. Sci. 7: 689–695.

Vikas P.A., Sajeshkumar N.K., Thomas P.C. 2012. Aquaculture related invasion of the exotic Artemia franciscana and displacement of the autochthonous Artemia populations from the hypersaline habitats of India. Hydrobiologia 684: 129–142.

Zheng B., Sun S.C. 2008. Taxonomic consideration of eight Chinese bisexual Artemia populations, based on the morphology of frontal knob and gonopod and the result of cross-breeding tests. Zootaxa 1919: 25–44.

Downloads

Published

2023-04-21

How to Cite

Biju, Y., Krishnakumar, S., & Krishnaveni, M. (2023). Morphometric and molecular sequencing evidence for the identification of Artemia franciscana (Kellogg, 1906) in Southern India. Environmental and Experimental Biology, 21(1), 21–26. https://doi.org/10.22364/eeb.21.03