Osmotic stress induced alterations in fatty acid composition and other metabolic responses in seedlings of Sinapis alba
DOI:
https://doi.org/10.22364/eeb.21.02Keywords:
fatty acids, lipid peroxidation, lipids, osmotic stress, proline, Sinapis alba, sugars, triacylglycerolAbstract
Sinapis alba L. (white mustard), an annual member of Brassicaceae, is an important oilseed crop with significant agronomic traits including tolerance to abiotic stress. The present study was aimed to assess the changes in biochemical attributes of S. alba, mainly the composition of lipids and fatty acids, in response to osmotic stress. Seedlings were subjected to stress condition by growing them on media supplemented with three different concentrations of polyethylene glycol (PEG 6000) solutions, respectively with ‑0.04, ‑0.16 and ‑0.64 MPa osmotic potential. Compatible solute content, lipid content, and lipid peroxidation of the seedlings were measured. Total fatty acid composition was analysed by gas chromatography mass spectrometry. Overall negative impact of PEG-mediated osmotic stress was apparent from seedling morphology. Total lipid content decreased in stressed plants together with increased level of triacylglycerol. Increase of lipid peroxidation in stressed condition indicated suppression of membrane integrity. The key outcome was the significant increase of unsaturation level of fatty acids due to higher accumulation of linoleic acid, α-linolenic acid and erucic acid in the seedlings exposed to higher osmotic stress. The results indicate that, in white mustard, the biochemical changes induced by osmotic stress mainly depend on changes in lipid composition and fatty acid unsaturation level, which maintain membrane stability during the early stage of seedling development.
References
Agrawal V.P., Stumpf P.K. 1985. Elongation systems involved in the biosynthesis of erucic acid from oleic acid in developing Brassica juncea seeds. Lipids 20: 361–366.
Aziz A., Larher F. 1998. Osmotic stress induced changes in lipid composition and peroxidation in leaf discs of Brassica napus L. J. Plant Physiol. 153: 754–762.
Bates L.S., Waldren, R.P., Teare, I.D. 1973. Rapid determination of free proline for water-stress studies. Plant Soil 39: 205–207.
Bavaro L., Catucci L., Depalo N., Ventrella A., Corcelli A., Agostiano A. 2007. Lipid content in higher plants under osmotic stress. Bioelectrochemistry 70: 12–17.
Bellaloui N., Reddy K.N., Mengistu, A. 2015. Drought and heat stress effects on soybean fatty acid composition and soil stability. In: Preedy V. (Ed.) Processing and Impact on Active Components in Food. Academic Press, Amsterdam, pp. 377–384.
Bewley J.D. 2001. Seed germination and reserve mobilization. eLS doi: 10.1038/npg.els.0002047.
Blake D.T., Diosady L.L. 2000. Rapid aqueous extraction of mucilage from whole white mustard seed. Food Res. Int. 33: 347–356.
Bligh E.G., Dyer W.J. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.
Blum A. 2005. Drought resistance, water-use efficiency, and yield potential—are they compatible, dissonant, or mutually exclusive? Austr. J. Agric. Res. 56: 1159–1168.
Bouchereau A., Clossais-Besnard N., Bensaoud A., Leport L., Renard M. 1996. Water stress effects on rapeseed quality. Eur. J. Agron. 5: 19–30.
Bray E.A. 1997. Plant responses to water deficit. Trends Plant Sci. 2: 48–54.
Christie W.W. 1993. Preparation of ester derivatives of fatty acids forchromatographic analysis. In: Christie W.W. (Ed.) Advances in Lipid Methodology. Oily Press, Dundee, pp. 69–111.
Ciubota-Rosie C., Macoveanu M., Fernandez C.M., Ramos M.J., Pérez A., Moreno A. 2013. Sinapis alba seed as a prospective biodiesel source. Biomass Bioenergy 51: 83-90.
Cserhalmi Z., Markus Z., Czukor B., Barath A., Toth M. 2000. Physico-chemical properties and food utilization possibilities of RF-treated mustard seed. Innov. Food Sci. Emerg. Technol. 1: 251–254.
Dakhma W.S., Zarrouk M., Cherif, A. 1995. Effects of drought-stress on lipids in rape leaves. Phytochemistry 40: 1383-1386.
Darko E., Vegh B., Khalil R., Marcek T., Szalai G., Pal M., Janda T. 2019. Metabolic responses of wheat seedlings to osmotic stress induced by various osmolytes under iso-osmotic conditions. PLoS One 14: e0226151.
Dornboss Jr. D.L., Mullen, R.E. 1992. Soybean seed protein and oil contents and fatty acid composition adjustments by drought and temperature. J. Amer. Oil Chem. Soc. 69: 228–231.
Downey R.K., Stringam G.R., McGregor D.I, Stefansson B.R. 1975. Breeding rapeseed and mustard crops. In: Harapiak J.T. (Ed.) Oilseed and Pulse Crops in Western Canada. Western Cooperative Fertilizers, Calgary.
Ehdaie B., Layne A.P., Waines J.G. 2012. Root system plasticity to drought influences grain yield in bread wheat. Euphytica 186: 219–232.
Feizabadi A., Noormohammadi G., Fatehi F. 2020. Changes in growth, physiology, and fatty acid profile of rapeseed cultivars treated with vermicompost under drought stress. J. Soil Sci. Plant Nutr. 21: 200–208.
Galliard T. 1979. The enzymatic degradation of membrane lipids in higher plants. In: Appleqvist L.A., Liljenberg C. (Eds.) Advances in the Biochemistry and Physiology of Plant Lipids. Elsevier, Amsterdam, pp. 121–132.
Gigon A., Matos A., Laffray D., Zuily-Fodil Y., Pham-Thi, A. 2004 Effect of drought stress on lipid metabolism in the leaves of Arabidopsis thaliana (ecotype Columbia). Ann. Bot. 94: 345–351.
Guo Y.Y., Yu H.Y., Yang M.M., Kong D.S., Zhang Y.J. 2018. Effect of drought stress on lipid peroxidation, osmotic adjustment and antioxidant enzyme activity of leaves and roots of Lycium ruthenicum Murr. seedling. Rus. J. Plant Physiol. 65: 244–250.
Halder K.P., Burrage, S.W. 2003. Drought stress effects on water relations of rice grown in nutrient film technique. Pak. J. Biol. Sci. 6: 441-444.
Hammer O., Harper D.A.T., Ryan P.D. 2001. PAST: Paleontological statistics software package for education and data analysis. Paleontol. Electron. 4: 1–9.
Hanson A.D., Hitz W.D. 1982. Metabolic responses of mesophytes to plant water deficits. Annu. Rev. Plant Physiol. 33: 162–203.
He M., Ding, N.Z. 2020. Plant unsaturated fatty acids: Multiple roles in stress response. Front. Plant Sci. 11: 562785.
Heath R.L., Packer L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125: 189–198.
Hemmati K., Ebadi A., Khomari S., Sedghi M. 2018. Influence of ascorbic acid and 24-epibrassinolide on physiological characteristics of pot marigold under water-stress condition. J. Plant Interact. 13: 364–372.
Hubac C., Guerrier D., Ferran J., Tremolieres, A. 1989. Changes of leaf lipid composition during water stress in two genotypes of Lupinus albus resistant or susceptible to drought. Plant Physiol. Biochem. 27: 739–744.
Iba K. 2002. Acclimative response to temperature stress in higher plants: Approaches for gene engineering for temperature tolerance. Annu. Rev. Plant Biol. 53: 225–245.
Krzymanski J., Downey R.K. 1969. Inheritance of fatty acid composition in winter forms of rapeseed Brassica napus. Can. J. Plant Sci. 49: 313–319.
Liu K.S., Brown, E.A. 1996. Fatty acid compositions in newly differentiated tissues of soybean seedlings. J. Agri. Food Chem. 44: 1395–1398.
MaY., Dias M.C., Freitas H. 2020. Drought and salinity stress responses and microbe-induced tolerance in plants. Front. Plant Sci. 11: 591911.
Matos A.R, d’Arcy-Lameta A., Franca M., Petres S., Edelman L., Kader J., Zuily-Fodil Y., Pham-Thi A.T. 2001. A novel patatin-like gene stimulated by drought stress encodes a galactolipid acyl hydrolase. FEBS Lett. 491: 188–192.
Meneses C.H.S.G., Bruno R.D.L.A., Fernandes P.D., Pereira W.E., Lima L.H.G.D.M., Lima M.M.D.A., Vidal M.S. 2011. Germination of cotton cultivar seeds under water stress induced by polyethyleneglycol-6000. Sci. Agric. 68: 131–138.
Michel B.E., Kaufman M.R. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914–916.
Mickky B.M., Aldesuquy H.S. 2017. Impact of osmotic stress on seedling growth observations, membrane characteristics and antioxidant defense system of different wheat genotypes. Egypt. J. Basic Appl. Sci. 4: 47–54.
Mitrović P.M., Stamenković O.S., Banković-Ilić I., Djalović I.G., Nježić Z.B., Farooq M., Siddique K.H.M., Veljković V.B. 2020. White mustard (Sinapis alba L.) oil in biodiesel production: a review. Front. Plant Sci. 11: 299.
Monteiro de Paula F., Pham Thi A.T., Viera da Silva J., Justin A.M., Demaudre C., Maziliak P. 1990. Effects of water stress on the molecular species composition of polar lipids from Vigna unguiculata L. leaves. Plant Sci. 66: 185–193.
Montillet J.L., Agnel J.P., Ponchet M., Vailleau F., Roby D., Triantaphylidès C. 2002. Lipoxygenase-mediated production of PUFA hydroperoxydes is a specific signature of the hypersensitive reaction in plants. Plant Physiol. Biochem. 40: 633–639.
Muscolo A., Sidari M., Anastasi U., Santonoceto C., Maggio A. 2014. Effect of PRG-induced drought stress on seed germination of four lentil genotypes. J. Plant Interact. 9: 354–363.
Navari-Izzo F., Quartacci M.F., Melfi D., Izzo R. 1993. Lipid composition and plasma membranes isolated from sunflower seedlings grown under water-stress. Physiol. Plant. 87: 508-514.
Offem J.O., Egbe E.O., Onen A.I. 1993. Changes in lipid content and composition during germination of groundnuts. J. Sci. Food Agri. 62: 147–155.
Phutela A., Jain V., Dhawan K., Nainawatee H.S. 2000. Proline metabolism under water stress in the leaves and roots of Brassica juncea cultivars differing in drought tolerance. J. Plant Biochem. Biotechnol. 9: 35–39.
Quinn P.J., Joo F., Vigh L. 1989. The role of unsaturated lipids in membrane structure and stability. Prog. Biophys. Mol. Biol. 53: 71–103.
Ranjbarfordoei A., Samson R., Van Damme P., Lemeur R. 2000. Effects of drought stress induced by polyethylene glycol on pigment content and photosynthetic gas exchange of Pistacia khinjuk and P. mutica. Photsynthetica 38: 443–447.
Rhodes D., Samaras Y. 1994. Genetic control of osmoregulation in plants. In: Strange K. (ed) Cellular and Molecular Physiology of Cell Volume Regulation. CRC Press, Boca Raton, pp. 347–367.
Sánchez-Martín J., Mur L.A.J., Rubiales D., Prats E. 2012. Targeting sources of drought tolerance within an Avena spp. collection through multivariate approaches. Planta 236: 1529–1545.
Sharafi Y., Majidi M.M., Goli S.A.H., Rashidi F. 2015. Oil content and fatty acid composition of Brassica species. Int. J. Food Prop. 18: 2145–2154.
Sobrino E., Ana M., Tarquis M., Díaz C. 2003. Modeling the oleic acid content in sunflower oil. Agron. J. 95: 329–334.
Toosi A.F., Bakar B.B., Azizi M. 2014. Effect of drought stress by using PEG 6000 on germination and early seedling growth of Brassica juncea var. ensabi. Agronomy 47: 360–363.
Toumi I., Gargouri M., Nouairi I., Moschou P.N., Salem-Fnayou A.B., Mliki A., Zarrouk M., Ghorbel A. 2008. Water stress induced changes in the leaf lipid composition of four grapevine genotypes with different drought tolerance. Biol. Plant. 52: 161–164.
Upchurch J.D. 2008. Fatty acid unsaturation, mobilization, and regulation in the response of plants to stress. Biotechnol. Lett. 30: 967-977.
Weatherley P.E. 1950. Studies in the water relations of the cotton plant: I. The field measurement of water deficits in leaves. New Phytol. 49: 81–97.
Yaniv Z., Schafferman D., Elber Y., Ben-Moshe E., Zur M. 1994. Evaluation of Sinapis alba, native to Israel, as a rich source of erucic acid in seed oil. Ind. Crop. Prod. 2: 137–142.
Yemm E.W., Willis A.J. 1954. The estimation of carbohydrates in plant extracts by anthrone. Biochem. J. 57: 508–514.
Yoshiba Y., Kiyosue T., Nakashima K., Yamaguchi-Shinozaki K., Shinozaki K. 1997. Regulation of levels of proline as an osmolyte in plants under water stress. Plant Cell Physiol. 38: 1095–1102.
Zhang M., Barg R., Yin M., Gueta-Dahan Y., Leikin-Frenkel A., Salts Y., Shabtai S., Ben-Hayyim G. 2005. Modulated fatty acid desaturation via overexpression of two distinct omega-3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants. Plant J. 44: 361–371.
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