Valorization of olive mill wastewater for acetic acid production by Bacillus strains isolated from bovine rumen
DOI:
https://doi.org/10.22364/eeb.23.03Keywords:
acetic acid, Bacillus, bovine rumen, olive mill wastewater, wastewater valorizationAbstract
The valorization of olive mill wastewater (OMW) through microbial fermentation presents an innovative approach to addressing environmental challenges associated with olive oil production. This study aimed to investigate the potential of Bacillus strains isolated from bovine rumen for acetic acid production using olive oil mill wastewater as the primary substrate. Physicochemical analyses revealed high organic load (chemical oxygen demand of 183 g O₂ L–1, biological oxygen demand of 7 g O₂ L–1) and acidic pH (4.5) in olive oil mill wastewater, making it suitable for microbial growth. A total of 25 bacterial strains were isolated, and preliminary screening based on biogas production identified five efficient acid-producing Bacillus strains. Species-level identification using the bacterial identification system confirmed the presence of Bacillus licheniformis and Bacillus circulans. Batch fermentations conducted over 120 h produced up to 14 mL of biogas per 100 mL of culture and acetic acid concentrations of 28 g L–1, highlighting the strains’ strong acidification capacity. This study demonstrates the feasibility of bioconverting agricultural waste into valuable bioproducts, contributing to sustainable waste management, bioenergy generation, and promoting circular economy practices.
References
Al Rabadi S., Alzboon K., Shawaqfah M., Damseh R., Alzoubi O. 2021. Biogas production through co-digestion of olive mill with municipal sewage sludge and cow manure. Environ. Nat. Resour. J. 20: 137–147.
Angeloni G., Spadi A., Corti F., Calcaprina M., Carpi G., Maioli F., Parenti A., Masella P. 2024. Advancing circular economy in olive oil production: Comparing maturation systems for vermicompost creation from olive pomace. Biomass 4: 65.
APHA. 2017a. Biochemical oxygen demand (BOD). In: Standard methods for the examination of water and wastewater. American Public Health Association.
APHA. 2017b. Chemical oxygen demand (COD). In: Standard methods for the examination of water and wastewater. American Public Health Association.
Ayadi K., Meziane M., Rouam D., Bouziane M.N., El-Miloudi K. 2022. Olive mill wastewater for bioethanol production using immobilised cells. Kem. Ind. 71: 21–28.
Bougherara S., Boucetta N., Lecheb F., Medjdoub F., Delleci K., Belkhir M. 2021. Characterization and valorisation of olive by-products from a traditional oil mill in Béjaia. Alger. J. Environ. Sci. Technol. 7: 1819–1824.
Bouharat D., Nabila S.A., Souhail B., Laghdach A., El Yousfi F. 2018. Physico-chemical characterization of olive-oil mill wastewaters of Ben Karrich area (Tetouan province, North of Morocco) and optimization study of their treatment using activated carbon. Mediterr. J. Chem. 7: 253–258.
Bouknana D., Hammouti B., Salghi R., Jodeh S., Zarrouk A., Warad I., Aouniti A., Sbaa M. 2014. Physicochemical characterization of olive oil mill wastewaters in the eastern region of Morocco. J. Mater. Environ. Sci. 5: 1039–1058.
Carmona I., Aguirre I., Griffith D.M., García-Borrego A. 2023. Towards a circular economy in virgin olive oil production: Valorization of the olive mill waste (OMW) “alpeorujo” through polyphenol recovery with natural deep eutectic solvents (NADESs) and vermicomposting. Sci. Total Environ. 872: 162198.
De Leonardis A., Masino F., Macciola V., Montevecchi G., Antonelli A., Marconi E. 2019. A study on acetification process to produce olive vinegar from oil mill wastewaters. Eur. Food Res. Technol. 245: 2123–2131.
Fronteras J.P., Dacera D.D.M., Bello D.D., Delos Santos K.J.L. 2021. Utilization of pesticide-free calamansi (Citrus microcarpa) and mango (Mangifera indica) peels for the production of acetic acid with potential industrial application. Bioresour. Technol. Rep. 15: 100806.
Graham E.B., Knelman J.E. 2023. Implications of soil microbial community assembly for ecosystem restoration: Patterns, process, and potential. Microbiol. Ecol. 85: 809–819.
Gueboudji Z., Addad D., Kadi K., Nagaz K., Secrafi M., Yahya L.B., Lachehib B., Abdelmalek A. 2022. Biological activities and phenolic compounds of olive oil mill wastewater from Abani, endemic Algerian variety. Sci. Rep. 12: 6042.
Laabidi F., Besbès R., Jamoussi B. 2023. Production of biogas from olive mill waste waters treated by cow manure. Green Sustain. Chem. 13: 62–71.
Liew F., Martin M.E., Tappel R.C., Heijstra B.D., Mihalcea C., Köpke M. 2016. Gas fermentation – a flexible platform for commercial scale production of low-carbon-fuels and chemicals from waste and renewable feedstocks. Front. Microbiol. 7: 694.
Mizrahi I., Wallace R.J., Moraïs S. 2021. The rumen microbiome: Balancing food security and environmental impacts. Nat. Rev. Microbiol. 19: 553–566.
Ntougias S., Bourtzis K., Tsiamis G. 2013. The microbiology of olive mill wastes. BioMed Res. Int. 2013: 784591.
Park S.A., Bhatia S.K., Park H.A., Kim S.Y., Sudheer P.D.V.N., Yang Y.H., Choi K.Y. 2021. Bacillus subtilis as a robust host for biochemical production utilizing biomass. Crit. Rev. Biotechnol. 41: 827–848.
Qiu X, Zhang Y, Hong H. 2021. Classification of acetic acid bacteria and their acid resistant mechanism. AMB Express 11: 29.
Russo E., Spallarossa A., Comite A., Pagliero M., Guida P., Belotti V., Caviglia D., Schito A.M. 2022. Valorization and potential antimicrobial use of olive mill wastewater (OMW) from Italian olive oil production. Antioxidants 11: 5.
Sar T., Akbas M. 2023. Antimicrobial activities of olive oil mill wastewater extracts against selected microorganisms. Sustainability 15: 8179.
Senanayake D., Torley P.J., Chandrapala J., Terefe N.S. 2023. Microbial fermentation for improving the sensory, nutritional and functional attributes of legumes. Fermentation 9: 7.
Serin B., Akcan N., Uyar F. 2012. Production and optimization of α-amylase from Bacillus circulans ATCC 4516 with solid state fermentation. Hacettepe J. Biol. Chem. 40: 393–400.
Shleeva M.O., Kondratieva D.A., Kaprelyants A.S. 2023. Bacillus licheniformis: A producer of antimicrobial substances, including antimycobacterials, which are feasible for medical applications. Pharmaceutics 15: 1893.
Sode F. 2014. Simultaneous determination of peracetic acid and acetic acid by titration with NaOH. Anal. Methods 6: 2406-2409.
Tamang J.P., Watanabe K., Holzapfel W.H. 2016. Review: diversity of microorganisms in global fermented foods and beverages. Front. Microbiol. 7: 377.
Vavouraki A., Zakoura M., Dareioti M., Kornaros M. 2020. Biodegradation of polyphenolic compounds from olive mill wastewaters (OMW) during two-stage anaerobic co-digestion of agro-industrial mixtures. Waste Biomass Valor. 11: 5783–5791.
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